赵卉
beat365官方网站，云南生物资源保护与利用国家重点实验室
博士，研究员，zhaohui@ynu.edu.cn
云南省中青年学术和技术带头人，
昆明市中青年学术和技术带头人。
主持国家自然科学基金项目5项。研究成果在Nanoscale、Nucleic Acids Res、Sci Bull、Biosens Bioelectron、Sens Actuators B Chem 等国际刊物上发表SCI论文37篇, 其中中科院JCR 1区论文15篇，Web of Science高被引论1篇，授权发明专利5项。
研究领域与方向
疾病分子机制研究和疾病相关生物标志物的检测
教育经历
2001-04 至 2005-03, 日本山口大学, 博士（硕博连读）
1994-09 至 1999-06, 中央民族大学, 学士
科研与学术工作经历
1）2017-12 至今，beat365官方网站，云南生物资源保护与利用国家重点实验室, 研究员
2）2007-02 至 2017-11, beat365官方网站，云南生物资源保护与利用国家重点实验室, 副研究员
3）1999-09 至 2001-03, 云南农业大学, 食品科学学院, 助教
获奖和名誉称号
1）国家自然科学基金委员会，地区科学基金项目，32160236，基于共价框架材料的CRISPR电化学传感器的构建及其对非小细胞肺癌ctDNA的检测研究，2022-01-01 至 2025-12-31，40万元，主持
2）国家自然科学基金委员会，地区科学基金项目，31760311，人类TBC1D8B基因两个转录本在癌症中的作用机制研究，2018-01-01 至 2021-12-31，39万元，主持
3）云南省科学技术厅云南省应用基础研究计划项目，重点项目，2018FA005，基于超分子主体/金属氧化物纳米复合体系的生物传感器的构建及其在癌症非编码RNA检测中的应用，2018-06至2021-05，50万元，参加
4）国家自然科学基金委员会，地区科学基金项目，21565029，基于超分子主体/鳞片状MnO2纳米复合材料的电化学生物传感器的构建及其在新型肿瘤标志物检测中的应用，2016-01至2019-12，48万元，参加
5）国家自然科学基金委员会，地区科学基金项目，31460038，microRNA对黄牛和水牛朊病毒基因转录后调控机制的研究，2015-01至2018-12，53万元，主持
6）国家自然科学基金委员会，地区科学基金项目，31260032，黄牛Sho疏水结构域多态对疯牛病调控蛋白功能的影响，2013-01至2016-12，46万元，主持
7）国家自然科学基金委员会，地区科学基金项目，31060302，中国水牛不易感疯牛病的遗传机制研究，2011-01至2013-12，23万元，主持
8）云南省科学技术厅云南省应用基础研究计划项目，面上项目，2010CD010，中国黄牛疯牛病易感性遗传机制研究，2010-01至2013-12，7.5万元，主持
9）中华人民共和国教育部留学回国人员科研启动基金，云南黄牛和水牛的Prion基因多样性的研究，2009-01至2011-12，2.5万，主持
主持或入选人才项目
1）2021年指导项目荣获第十七届全国“挑战杯”竞赛“黑科技”专项赛“卫星”级奖
2）2021年指导项目荣获第七届云南省“互联网+”大学生创新创业大赛省级金奖
3）2020年指导项目荣获第六届云南省“互联网+”大学生创新创业大赛省级銀奖
4）2020年荣获“云南省中青年学术和技术带头人”称号
5）2019年荣获“云南省优秀硕士论文指导奖”
6）2018年入选beat365官方网站“青年英才培育”项目
7）2017年荣获“云南省优秀硕士论文指导奖”
8）2017年入选“昆明市中青年学术和技术带头人”
9）2016年荣获“云南省优秀硕士论文指导奖”
10）2015年荣获“beat365官方网站伍达观教育基金优秀教师奖”
代表性论文和专著情况（“#”表示所有共同第一作者，“*”表示通讯作者及共同通讯作者）
1) Zhao H #, Liu F#, Xie W#, Zhou TC#, Ouyang J, Jin L, Li H, Zhao CY, Zhang L, Wei J*, Zhang YP*, Li CP*. Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone. Sens. Actuators: B. Chem., 2021, 327: 128899. (中科院JCR区) Web of Science高被引论文
2) Zhao H#, Xie# W, Zhang RL#, Wang XD, Liu HF, Li J, Sha T, Guo XS, Li J, Sun QM*, Zhang YP*, Li CP*. Electrochemical sensor for human norovirus based on covalent organic framework/pillararene heterosupramolecular nanocomposites. Talanta. 2022, 237: 122896.
3) Zheng J#, Zhao H#, Ning GB, Sun WJ, Wang L, Ling H, Xu HB, He CY, Zhao H*, Li CP*. A novel affinity peptide-antibody sandwich electrochemical biosensor for PSA based on the signal amplication of MnO2-functionalized covalent organic framework. Talanta. 2021, 233: 122520.
4) Li CP, Liu F, Zheng J, Zhao H*. A novel electrochemical assay for chymosin determination using a label-free peptide as a substrate. J Dairy Sci. 2021, 104: 2511-2519.
5) Li CP#, Lu YX#, Zi CT, Zhao YT, Zhao H*, Zhang YP*. Cationic pillar[6]arene induces cell apoptosis by inhibiting protein tyrosine phosphorylation via host-guest recognition. Int J Mol Sci. 2020. 21(14): E4979.
6) Liang H#, Xu HB#, Zhao YT, Zheng J, Zhao H*, Li GL*, Li CP*. Ultrasensitive electrochemical sensor for prostate specific antigen detection with a phosphorene platform and magnetic covalent organic framework signal amplifier. Biosens Bioelectron. 2019. 144: 111691. (中科院JCR区)
7) Zhao H#, Liu F#, Lu YX, Jin L, Tan S, Zhang YP*, Li CP*. Ultrasensitive electrochemical detection of alternative cleavage and polyadenylation of CCND2 gene at single cell level. 2019. Sensors & Actuators: B. Chemical. 2019. 285: 553-561. (中科院JCR区)
8) Tan S#, Han R#, Wu SL, Liang H, Zhao YT, Zhao H*, Li CP*. A novel fluorescent sensing platform for insulin detection based on competitive recognition of cationic pillar[6]arene. Talanta. 2019. 1:130-137.
9) Li CP, Tan S, Ye H, Cao J, Zhao H* A novel fluorescence assay for resveratrol determination in red wine based on competitive host-guest recognition. Food Chem., 2019, 283, 191-198. (中科院JCR区)
10) Zhou ZY#, Hu Y#, Li A#, Li YJ#, Zhao H#, Wang SQ, Otecko NO, Zhang D, Wang JH, Liu Y, Irwin DM, Qin Y, Zhang YP*. Genome wide analyses uncover allele-specific RNA editing in human and mouse. Nucleic Acids Res. 2018: 46(17):8888-8897. (中科院JCR区)
11) Zhao H#, Liu F#, Wu SL, Yang L, Zhang YP*, Li CP*. Ultrasensitive electrochemical detection of Dicer1 3’UTR for the fast analysis of alternative cleavage and polyadenylation. Nanoscale. 2017. 9: 4272 - 4282. (中科院JCR区)
12) Zhao H#, Ma TF#, Lin J#, Liu LL, Sun WJ, Guo LX, Wang SQ, Otecko Newton O, Zhang YP*. Identification of valid reference genes for mRNA and microRNA normalisation in prostate cancer cell lines. Scientific Reports. 2018. 8:1949
13) Zhao H#, Wang SQ#, Guo LX#, Du YL, Liu LL, Ma TF, Newton O. Otecko, Li CP*, Zhang YP*. Fixed differences in the 3’UTR of buffalo PRNP gene provide binding sites for miRNAs post-transcriptional regulation. Oncotarget. 2017. 8(28): 46006-46019.
14) Zhao H#, Yang L#, Li YC, Ran X, Ye HZ, Zhao GF, Zhang YQ, Li CP*. A comparison study of macrocyclic hosts functionalized reduced graphene oxide for electrochemical recognition of tadalafil. Biosens Bioelectron. 2017. 89: 361-369. (中科院JCR区)
15) Ye LQ #, Zhao H #, Zhou HJ # , Ren XD # , Liu LL # , Otecko Newton O, Wang ZB, Yang MM, Zeng L, Hu XT, Yao YG, Zhang YP*, Wu DD*. The RNA editome of Macaca mulatta and functional characterization of RNA editing in mitochondria. Sci Bull. 2017. 62: 820–830. (中科院JCR区) 封面杂志文章
16) Yang L#, Zhao H#, Li Y, Zhang YC, Ye HZ, Zhao GF, Ran X, Liu F, Li C P*. Insights into the recognition of dimethomorph by disulfide bridged β–cyclodextrin and its high selective fluorescence sensing based on indicator displacement assay. Biosens Bioelectron. 2017, 87, 737-744. (中科院JCR区)
17) Zhao H#, Wang SQ#, Qing LL, Liu LL, Zhang YP*. Expression of BSE-associated proteins in the CNS and lymphoreticular tissues of cattle and buffalo. Sci Bull. 2016. 61(17): 1377-1383. (中科院JCR区)
18) Yang L, Xie XG, Cai L, Ran X, Li YC, Yin TP, Zhao H*, Li CP*. P-sulfonated calix[8]arene functionalized grapheme as a “turn on” fluorescent sensing platform for aconitine determination. Biosens Bioelectron. 2016. 82: 146-154. (中科院JCR区)
19) Yang L, Ran X, Cai L, Li YC, Zhao H*, Li CP*. Calix[8]arene functionalized single-walled carbon nanohorns for dual-signalling electrochemical sensing of aconitine based on competitive host-guest recognition. Biosens Bioelectron. 2016.83: 347-352. (中科院JCR区)
20) Yang L#, Zhao H#, Li YC, Ran X, Deng GG, Zhang YQ, Ye HZ, Zhao GF, Li CP. Indicator displacement assay for cholesterol electrochemical sensing using a calix[6]arene functionalized grapheme-modified electrode. Analyst. 2015. 141: 270-278.
21) Yang L#, Zhao H#, Li YC, Ran X, Deng GG, Xie XG, Li CP*. Fluorescent detection of tadalafil based on competitive host-guest interaction using p-sulfonated calix[6]arene functionalized grapheme. ACS Appl Mater Interfaces. 2015. 7: 26557-26565. (中科院JCR区)
22) Yang L#, Zhao H#, Deng GG, Ran X, Li YC, Li CP* Immunosensor for prostate-specific antigen using Au/Pd@flower-like SnO2 as platform and Au@mesoporous carbon as signal amplification. RSC Adv. 2015. 5: 74046-74053.
23) Yang L#, Zhao H#, Fan SM, Zhao GF, Ran X, Li CP.* Electrochemical detection of cholesterol based on competitive host–guest recognition using a β-cyclodextrin/poly(N-acetylaniline)/ graphene-modified electrode. RSC Adv. 2015. 5: 64146-64155.
24) Ran X#, Yang L#, Zhao G, Ye H, Zhang Y, Fan S, Xie XG,* Zhao H,* Li CP.* Simultaneous determination of two flavonoids based on disulfide linked β-cyclodextrin dimer and Pd cluster functionalized graphene-modified electrode. RSC Adv. 2015. 5: 60775-60785.
25) Zhao H#, Du YL#, Chen SM, Qing LL, Wang XY, Huang JF, Wu DD, Zhang YP*. The prion protein gene polymorphisms associated with bovine spongiform encephalopathy susceptibility differ significantly between cattle and buffalo. Infect Genet Evol. 2015. 36:531-538.
26) Liu LL#, Zhao H#, Ma TF, Ge F, Chen CS, Zhang YP*. Identification of valid reference genes for the normalization of RT-qPCR expression studies in human breast cancer cell lines treated with and without transient transfection. PLoS ONE. 2015. 10(1): e0117058.
27) Yang L#, Zhao H#, Li CP*, Fan S, Li B. Dual β-cyclodextrin functionalized Au@SiC nanohybrids for the electrochemical determination of tadalafil in the presence of acetonitrile. Biosens Bioelectron. 2015. 64: 126-130. (中科院JCR区)
28) Qing LL, Zhao H*, Liu LL. Progress on low susceptibility mechanisms of transmissible spongiform encephalopathies. Zoological Research. 2014. 35(5): 436-445.
29) Yang L#, Zhao H#, Fan S, Deng S, Lv Q, Lin J*, Li CP* Label-free electrochemical immunosensor based on gold-silicon carbide nanocomposites for sensitive detection of human chorionic gonadotrophin. Biosens Bioelectron. 2014. 57: 199-206. (中科院JCR区)
30) Wang SQ, Zhao H*, Zhang YP. Advances in research on Shadoo, Shadow of prion protein. Chinese Sci Bull. 2014. 59(9): 821–827.
31) Zhao H#, Liu LL#, Du SH, Wang SQ, Zhang YP*. Comparative analysis of the Shadoo gene between cattle and buffalo reveals significant differences. PLoS One. 2012. 7(10): e46601.
32) Zhao H, Mitsuiki S*, Takasugi M, Sakai M, Goto M, Kanouchi H, Oka T. Decomposition of insoluble and hard-to-degrade animal protein by enzyme E77 and its potential applications. Appl Biochem Biotechnol. 2012. 166(7):1758-1768.
33) Zhao H, Wang XY, Zou W, Zhang YP*. Prion protein gene (PRNP) polymorphisms in native Chinese cattle. Genome. 2010. 53:138-145.
34) Zhao H, Wang XY, Zhang YP*. The indel polymorphisms of prion protein gene (PRNP) in Chinese yak. Anim Genet. 2009. 40: 575-576.
35) Mitsuiki S, Zhao H, Matsumoto D, Sakai M, Moriyama Y, Furukawa K, Kanouchi H, Oka T. Degradation of PrPSc by keratinolytic protease from Nocardiopsis sp. TOA-1. Biosci Biotechnol Biochem. 2006. 70:1246-1248.
36) Zhao H, Doi H, Kanouchi H, Matsuura Y, Mohri S, Nonomura Y, Oka T. Alkaline serine protease produced by Streptomyces sp degrades PrPSc. Biochem Biophys Res Comm. 2004. 321: 45-50.
37) Zhao H, Minamiguchi K, Doi H, Kinoshita N, Kanouchi H, Oka T. Recombinant alkaline serine protease II degrades PrPSc. In Vitro Cell Dev-An. 2004. 40: 293-296.
38) 卢宇勋，李灿鹏*，赵卉*。基于电化学生物传感器的核酸肿瘤标志物检测研究进展。中国科学：生命科学。2019. 49(7): 814-827.
39) 郭丽霞#，孙伟杰#，赵卉*。可选择性多聚腺苷酸化与肿瘤的相关研究进展。昆明医科大学学报。2019. 40(9): 130-135.
招生方向
动物学专业、生物与医药专业（动物学方向）